Development and Retranslational Validation of an In Vitro Model to Characterize Acute Infections in Large Human Joints
Figure 6
Viability and functionality of chondrocytes (CHDR). Cocultivated CHDR exposed to LPS, LPS PMA, or unchallenged were tested for their viability by MTS (a). While the LPS challenge tends to increase metabolic activity in CHDR, prolonged and severe inflammatory challenge by LPS PMA tend to result in diminished metabolic activity. However, we observed no significant differences in viability (a). Histological analyses by Alcian-Blue HCl staining for highly sulphated glycoproteins support these findings ((b) left: control; middle: LPS; right: LPS PMA challenge). Statistics: (a) Student’s t-test. Scale bar 50 μm.
