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Figure 9: Atg6 and the other members of PI3K (III) are required for normal wing development: (a), (f) 1-day-old wild type flies. (b) Newly hatched Atg6 RNAi animals developed serious malformations, blisters in their wings. (c), (d) In 1-day-old Atg6 RNAi animals, the blisters had become collapsed; thus the wings were creased and heavily distorted. (g), (h) The RNAi of UVRAG completely mimiced the effect of Atg6 RNAi. (e), (f) Positive control animals: Vps34 RNAi and Vps15 RNAi flies, respectively. (j) Unlike the other members of PI3K (III) complex, the RNAi of Atg14 causes a vestigial-like morphology rather than blistering or creasing. (k) The Atg6 null mutant wings are heavily distorted and creased, (l) compared to the control wings lacking the mutation or (m) expressing an Atg6 transgene. 1 h: 1 hour; 1 d: 1 day after emerging from puparia, respectively. For genotypes, see Table S4.