Therapeutic Effects of PADRE-BAFF Autovaccine on Rat Adjuvant Arthritis
Figure 2
Production and identification of PADRE-BAFF. (a) Growth curve of engineered bacteria pQE30-PADRE-BAFF/BL21 (DE3) in fed-batch fermentation. (b) Expression and purification of PADRE-BAFF in E. coli. Lane 1, standard protein marker; lane 2, total bacterial proteins without induction; lane 3, total bacterial proteins with 1 mM IPTG induction; lanes 3–6, purification of PADRE-BAFF by chromatography. (c) Analysis of PADRE-BAFF by Western blot. Lane M, protein marker; lane 1, total proteins of pQE30-PADRE-BAFF/BL21 cells without induction; lane 2, total proteins of IPTG induced pQE30-PADRE-BAFF/BL21 cells. (d) Analysis of PADRE-BAFF by SEC-HPLC.