Adrenomedullin Deficiency and Aging Exacerbate Ischemic White Matter Injury after Prolonged Cerebral Hypoperfusion in Mice

Figure 4

Oxidative stress after prolonged cerebral hypoperfusion in young groups. ((a)–(d)) Photomicrographs and the number of 8OHdG- ((a), (b)) and HHE- ((c), (d)) positive cells in the corpus callosum at day 28 after BCAS. Scale bars = 20 μm. ((e), (f)) Double immunofluorescence staining for 8OHdG (green), HHE (green), GSTπ (red), and merged images in the corpus callosum at day 28 after reperfusion. Scale bars = 20 μm. ((g), (h)) Western blotting (g) and densitometric analysis (h) of oxidized proteins. Data are mean ± SEM of five mice in each group. ^∗𝑃<0.05, ^∗∗𝑃<0.001, compared with the preoperation group (pre). ^†𝑃<0.05, ^††𝑃<0.001, compared with the same time points. Pre: preoperation.

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