Modulation of c-Fos and BDNF Protein Expression in Pentylenetetrazole-Kindled Mice following the Treatment with Novel Antiepileptic Compound HHL-6
Figure 4
(a) Photomicrograph of c-Fos immunoreactivity in cortical region of normal and kindled mice brains with stage 5 seizure activity. Note the increased and scattered immunofluorescence in the PTZ-kindled control brain section. In contrast, the immunoreactivity of c-Fos in the brain of the kindled animals treated with HHL-6 and diazepam was markedly reduced. (b) Graphical representation of the level of expression of immunohistochemically detected c-Fos in kindled and unkindled brain sections analyzed by ImageJ software. In comparison to the normal control group (), the PTZ-control animals showed a significant increase in the c-Fos staining intensity (, *). In contrast, c-Fos expression in the kindled animals treated with HHL-6 (, **) and diazepam (, ) declines significantly.
