Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes
Figure 4
Dose response curve of the in vitro effects of VB, added during IVM culture, on mt activity and intracellular ROS levels in single prepubertal lamb MII stage oocytes vitrified/warmed, expressed as MitoTracker Orange CMTM Ros (panel (a)) and DCF (panel (b)) fluorescence intensities. Oocytes treated with 1.03 μM VB showed significantly higher intracellular ROS levels compared with controls. Values are expressed as ADU (arbitrary densitometric units). Pearson’s correlation coefficients of MitoTracker Orange CMTM Ros and DCF fluorescent labelling colocalization in MII oocytes obtained after IVM culture in presence of VB and vitrified/warmed (panel (c)). Numbers of analyzed oocytes per group are indicated at the bottom of each histogram. (a) One-way ANOVA: NS. (b) One-way ANOVA: a,b: . (c) One-way ANOVA: NS.
