Tumorigenic Factor CRIPTO-1 Is Immunolocalized in Extravillous Cytotrophoblast in Placenta Creta
Expression of CRIPTO-1 and cell markers in creta placentas. (a) Representative histological sections demonstrating immunolocalization of cytokeratin (CK: A–C), CRIPTO-1 (CR-1: D–F), and vimentin (Vm: G–I) in representative cases of accreta (A, D, G, and J), increta (B, E, H, and K) and percreta (C, F, I, and L) placentas. The arrowheads indicate cells reactive to cytokeratin and CRIPTO-1 in semiserial histological sections. Arrows depict vimentin-positive cells. ((c), J–L) Negative control of the immunohistochemistry reactions in which the respective primary antibody has been omitted. Immunoperoxidase, Mayer’s hematoxylin counterstaining. Bar in ((a)(A)) = 100 μm in all figures. (b-c) Quantification of the immunoreactivity (pixels/μm2) for cytokeratin (CK) and CRIPTO-1 (CR-1) proteins at the maternal-fetal interface in placentas from healthy mothers (gestation week 36) and accreta placentas (b) and of healthy placentas (gestation week 38) and increta and percreta placentas (c). Different superscript letters above the bars indicate the group statistically analyzed; means with different numbers are significantly different, , whereas means with similar numbers do not differ. Asterisks indicate significant differences in relation to CK in the same group (). The results of the analysis are given in the text.