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BioMed Research International
Volume 2014, Article ID 897361, 7 pages
http://dx.doi.org/10.1155/2014/897361
Research Article

Cytotoxicity of Portuguese Propolis: The Proximity of the In Vitro Doses for Tumor and Normal Cell Lines

1Centro de Investigação de Montanha (CIMO), ESA, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 1172, 5301-855 Bragança, Portugal
2Centro de Química, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal

Received 27 February 2014; Accepted 19 May 2014; Published 1 June 2014

Academic Editor: H. Kim

Copyright © 2014 Ricardo C. Calhelha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

With a complex chemical composition rich in phenolic compounds, propolis (resinous substance collected by Apis mellifera from various tree buds) exhibits a broad spectrum of biological activities. Recently, in vitro and in vivo data suggest that propolis has anticancer properties, but is the cytoxicity of propolis specific for tumor cells? To answer this question, the cytotoxicity of phenolic extracts from Portuguese propolis of different origins was evaluated using human tumor cell lines (MCF7—breast adenocarcinoma, NCI-H460—non-small cell lung carcinoma, HCT15—colon carcinoma, HeLa—cervical carcinoma, and HepG2—hepatocellular carcinoma), and non-tumor primary cells (PLP2). The studied propolis presented high cytotoxic potential for human tumor cell lines, mostly for HCT15. Nevertheless, excluding HCT15 cell line, the extracts at the GI50 obtained for tumor cell lines showed, in general, cytotoxicity for normal cells (PLP2). Propolis phenolic extracts comprise phytochemicals that should be further studied for their bioactive properties against human colon carcinoma. In the other cases, the proximity of the in vitro cytotoxic doses for tumor and normal cell lines should be confirmed by in vivo tests and may highlight the need for selection of specific compounds within the propolis extract.

1. Introduction

Epidemiological data support the concept that natural products, in special, phenols and polyphenols in diet are safe and nontoxic and have long-lasting beneficial effects on human health. Among the many biological properties presented by them, the ones involved with cancer therapy are remarkable [1].

Many plants rich in polyphenols have been shown to display interesting anticancer effects on cell lines and murine models, yielding higher effects when compared to pure natural or synthetic compounds [1]. Products originating in the beehive, such as honey, pollen, royal jelly, and propolis, are attractive both as ingredients for healthy foods and as medicinal products in apitherapy [2, 3].

Propolis is a chemically complex product obtained by bees from the resinous exudates of leaf buds, shoots, and petioles of leaves of different plants present around the hive, which is mixed with wax and salivary secretions. In the hive it has a multifunctional role as a material for construction, maintenance, and defense [4]. Named as bee glue, its chemical composition is highly variable and strongly dependent on the plant sources available around the hive. Furthermore, the propolis composition is dependent on the seasonality, altitude, collector type, food availability, and activity developed during propolis exploitation [5]. More than 300 chemical compounds were identified in propolis from different regions [6, 7]. The main chemical classes and most bioactive compounds found in propolis are the phenolic compounds, including phenolic acids, flavonoids, and their derivatives [6, 8].

Propolis has a wide spectrum of biological and pharmacological properties, which have been intensively researched in cell and animal experiments and thus it can be considered as a drug from the beehive [3]. Recently, studies concerning propolis and cancer have been attracting researchers [911]. Propolis and its components, namely, caffeic acid, caffeic acid phenethyl ester (CAPE), 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), and others, are frequently mentioned in the literature as antitumor and immunomodulatory agents [9, 10, 12]. Through in vitro and in vivo assays it was possible to define different mechanisms of actions for the bee glue and its components, such as suppressing cancer cells proliferation via its anti-inflammatory effects, decreasing the cancer stem cell populations, blocking specific oncogene signaling pathways, exerting antiangiogenic effects, and modulating the tumor microenvironment [911].

Propolis phenolic profile from different Portuguese regions was recently characterized and quantified through high-pressure liquid chromatography (HPLC) and liquid chromatography with diode-array detection coupled to electrospray ionization tandem mass spectrometry , providing identification of seventy-six phenolic compounds [13, 14]. Within the biological properties studied in Portuguese propolis, few studies deal with anticancer effects. Two samples from the northeast and center of Portugal strongly suppress the proliferation of primary renal cancer cells in a concentration-dependent manner [15]. Also, the in vitro antitumor activity of a propolis sample from Azores archipelago was evaluated on colon carcinoma cell line HCT15 presenting cytotoxicity in a dose- and time-dependent way [16].

Due to the complex nature of propolis which strongly depends on the local flora at the site of collection, for an effective evaluation of the anticancer properties of Portuguese propolis more regions have to be evaluated and the antitumor assays should be extended for other types of cell lines. Moreover, it is important to compare its cytotoxicity against tumor and normal cells. In this context, this work aims to study the growth inhibitory activity of phenolic extracts from Portuguese propolis of different regions on human tumor cell lines (MCF-7—breast adenocarcinoma, NCI-H460—non-small cell lung carcinoma, HCT15—colon carcinoma, HeLa—cervical carcinoma, and HepG2—hepatocellular carcinoma) and nontumor primary cells (PLP2).

2. Material and Methods

2.1. Samples
2.1.1. Samples Origin

The study was performed on propolis samples collected from five different geographical regions in Portugal: north (N1, Bragança; N8, Mirandela), central coast (CC3, Coruche), south (S2, Aljezur), Azores Archipelago (A2, S. Miguel Island), and Madeira Island (M1, Funchal). All the samples were obtained between 2007 and 2009 after the honey harvesting season (July/September), by conventional scraping or through plastic screens. These propolis samples were then stored at −20°C until analysis.

2.1.2. Phenolic Extracts Preparation

The extraction was made according to the work previously described [8]. Prior to the extraction, 1 g of powdered propolis sample was homogenized and mixed with 10 mL of 80% of ethanol/water and kept at 70°C for 1 h. The resulting mixtures were filtered and the residues were reextracted in the same conditions. After the second extraction, the filtrates were combined, concentrated, and freeze-dried. Complete chemical characterization of the phenolic extracts is available in Falcão et al. [13, 14] For cytotoxicity evaluation, the extracts were dissolved in DMSO at a final concentration of 8 mg/mL. The final solutions were further diluted to different concentrations. The results were expressed in GI50 (extract concentration that inhibited 50% of the net cell growth) values. Ellipticine was used as positive control.

2.2. Standards and Reagents

Fetal bovine serum (FBS), L-glutamine, Hank’s balanced salt solution (HBSS), trypsin-EDTA (ethylenediaminetetraacetic acid), penicillin/streptomycin solution (100 U/mL and 100 mg/mL, resp.), RPMI-1640, and DMEM media were from Hyclone (Logan, USA). Acetic acid, ellipticine, sulforhodamine B (SRB), trypan blue, trichloroacetic acid (TCA), and Tris were from Sigma Chemical Co. (Saint Louis, USA). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, USA).

2.3. Evaluation of Cytotoxicity in Human Tumour Cell Lines

Five human tumor cell lines were used: MCF-7 (breast adenocarcinoma) from DSMZ (Leibniz-Institut DSMZ—Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), NCI-H460 (non-small cell lung carcinoma), HCT15 (colon carcinoma), HeLa (cervical carcinoma), and HepG2 (hepatocellular carcinoma) from European collection of cell cultures (ECACC). Cells were routinely maintained as adherent cell cultures in RPMI-1640 medium containing 10% heat-inactivated FBS (MCF-7, NCI-H460, and HCT15) and 2 mM glutamine or in DMEM supplemented with 10% FBS, 2 mM glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin (HeLa and HepG2 cells), at 37°C, in a humidified air incubator containing 5% CO2. Each cell line was plated at an appropriate density ( cells/well for MCF-7, NCI-H460 and HCT15 or cells/well for HeLa and HepG2) in 96-well plates for 24 h. Cells were then treated for 48 h with various extract concentrations. Following this incubation period, the adherent cells were fixed by adding cold 10% trichloroacetic acid (TCA, 100 μL) and incubated for 60 min at 4°C. Plates were then washed with deionized water and dried; sulforhodamine B solution (0.1% in 1% acetic acid, 100 μL) was then added to each plate well and incubated for 30 min at room temperature. Unbound SRB was removed by washing with 1% acetic acid. Plates were air-dried, the bound SRB was solubilised with 10 mM Tris (200 μL) and the absorbance was measured at 540 nm in ELX800 Microplate Reader (Bio-Tek Instruments, Inc; Winooski, USA) [17].

2.4. Evaluation of Cytotoxicity in a Porcine Liver Primary Cell Culture

A cell culture was prepared from a freshly harvested porcine liver obtained from a local slaughter house, and it was designed as PLP2. Briefly, the liver tissues were rinsed in Hank’s balanced salt solution containing 100 U/mL penicillin and 100 μg/mL streptomycin and divided into  mm3 explants. Some of these explants were placed in 25 cm2 tissue flasks in DMEM medium supplemented with 10% fetal bovine serum, 2 mM nonessential amino acids, 100 U/mL penicillin, and 100 mg/mL streptomycin and incubated at 37°C with a humidified atmosphere containing 5% CO2. The medium was changed every two days. Cultivation of the cells was continued with direct monitoring every two to three days using a phase contrast microscope. Before confluence was reached, cells were subcultured and plated in 96-well plates at a density of cells/well and cultivated in DMEM medium with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin [17].

2.5. Statistical Analysis

For all the experiments three samples were analysed and all the assays were carried out in triplicate. The results are expressed as mean values and standard deviation (SD). The differences between the different samples were analysed using one-way analysis of variance (ANOVA) followed by Tukey’s honestly significant difference post hoc test with , coupled with Welch’s statistic. This treatment was carried out using SPSS v. 18.0 program. The regression analysis between phenolic acids, phenolic esters, total phenolics, flavonols, flavones, dihydroflavonols, flavanones, flavonoid esters, total flavonoids (mg/g of extract), and cytotoxicity GI50 values (μg/mL) was performed using the same statistical package.

3. Results

The cytotoxicity of the propolis phenolic extracts was evaluated in five human tumor cell lines (breast- MCF7, lung- NCI-H460, colon- HCT15, cervical- HeLa and hepatocellular- HepG2, carcinomas) and in a porcine liver primary cell culture (PLP2), established by us. All the studied extracts inhibited the growth of the mentioned tumor cell lines. N1 gave the highest cytotoxicity, followed by A2 (Table 1), presenting the lowest GI50 values against the tested tumor cell lines. The HCT15 cell line was the most sensible to the studied extracts, except to S2 extract, as the A2 extract was the most active (GI50 9 μg/mL; an excellent GI50 value in comparison with several natural extracts, for example, Guimarães et al. [17] This activity could be related to the chemical composition of this sample (Figure 1). From the analysis of Figure 1, it can be observed that samples N1 and A2 have major concentrations of total phenolics and phenolic acids, especially the sample N1.

tab1
Table 1: Cytotoxicity of phenolic extracts from Portuguese propolis of different origins (mean ± SD).
897361.fig.001
Figure 1: Phenolic classes presented in propolis of different Portuguese origins. [14].

Despite the lower concentration in total phenolics presented in the sample N8, the GI50 values for MCF7 cell line were not very different from the extracts N1 or A2, which can be due to the similar high content in total flavonoids and the specificity of this cell line to flavonoids. This sample presented the major concentration of flavones and flavanones, two important groups of compounds regarding biological activity.

The M1 sample from Madeira island showed the highest GI50 values for all the tested tumor cell lines. This fact could be explained by its poor chemical composition (Figure 1) that only includes flavones and in low concentration.

The correlations established between the chemical composition and the cytotoxicity results are presented in Table 2. The cytotoxicity observed for all the tested tumor cell lines, except MCF7, was positively correlated with phenolic acids ( values higher than 0.5) and, consequently, with total phenolic ( higher than 0.5) contents but not with phenolic esters ( lower than 0.5). NCI-H460 cell line presented the highest correlation for this class of compounds .

tab2
Table 2: Correlations established between content in phenolics and flavonoids (mg/g of extract) and cytotoxicity GI50 values (g/mL) of phenolic extracts from Portuguese propolis of different origins.

Total flavonoids were positively correlated ( values higher than 0.5) with the cytotoxicity observed against MCF7, NCI-H460, HCT15, and HeLa cell lines. However, the cytotoxicity was not correlated ( values lower than 0.5) with flavonols, dihydroflavonols, and flavonoid esters. The cytotoxicity observed in HCT15 cell line was the one that correlates better with the composition in phenolic compounds.

The evaluation of the cytotoxicity using PLP2 is very important since mammalian hepatocytes still represent an obligatory step in the evaluation of toxic compounds that lead to the production of various metabolites, which are the ultimate cause of toxicity. We used porcine liver as an in vitro cytotoxicity model because it is known, in terms of cellular and physiological functioning, to be very similar to human. Despite the high cytotoxicity displayed by most of the propolis samples against tumor cell lines, the samples also showed toxicity for nontumour (normal) liver primary culture (PLP2), with GI50 values close to those obtained for tumor cell lines. The cytotoxicity for PLP2 primary culture was only positively correlated with phenolic acids and consequently with total phenolics .

The colon carcinoma cells, HCT15, seem to be the most promising to be controlled by the studied propolis extracts since, with exception of sample S2, all the cytotoxicity levels for the tumor cells are significantly above the ones found for nontumor PLP2 cells. The same behaviour was observed for the NCI-H460 cell line; however, in this case the cytotoxicity levels for tumor and nontumor cells are close which reduces the selectivity. The sample from Madeira showed the lowest cytotoxicity for all the tested tumor cell lines, which indicates that the chemical composition of the samples has a significant influence on their bioactivity, since this sample presented a quite specific phenolic profile, only with flavones.

4. Discussion

The highest cytotoxicity revealed by samples N1 and A2 against all the tested tumor cell lines is certainly related to their higher concentrations in total phenolics and phenolic acids. Phenolic acids and analogues are, in fact, known to display a wide variety of biological functions, in addition to their primary antioxidant activity, which are mainly related to modulation of carcinogenesis [18, 19]. Indeed, many phenolic compounds have been investigated for their potential use as cancer chemopreventive agents [1, 20]. There are many reports on the bioactive constituents of propolis like cinnamic acid esters, such as caffeic acid phenethyl (CAPE), which is a component of propolis from honeybee hives, and benzyl esters that display selective antiproliferative, anticarcinogenic, and immunomodulatory properties [21, 22]. The molecular basis of this action may be connected to the inhibition of the nuclear transcription factor NK-kappa B [23]. Other authors refer that drupanin, baccharin, cinnamic acid derivatives inhibit the growth of HeLa-60 cell lines by inducing apoptosis [24].

The similar toxicity for MCF7 cell line observed between N8, N1, and A2 can be explained by the similar high content in total flavonoids, presenting N8 as the highest concentration of flavones and flavanones. The most common compounds within the flavones were apigenin, chrysin, and luteolin. They are responsible for main benefits to human health including anticarcinogenic effects [25]. In the flavanones group, hesperidin and naringin, the most important compounds, have also been reported as possessing anticancer properties [25].

The present data highlights the high cytotoxicity of Portuguese propolis against tumor cell lines, being in agreement with Búfalo et al. [26], who reported a marked activity of Brazilian green propolis against other tumor cells (human laryngeal epidermoid carcinoma (HEp-2)); the main mechanism seems to involve apoptosis [27]. Ethanol extract of Brazilian red propolis was also reported as promoting a significant reduction in MCF7 cell viability thought the induction of mitochondrial dysfunction, caspase-3 activity, and DNA fragmentation [28]. Moroccan propolis extracts (ethanolic and ethyl acetate) were described as exerting antiproliferative effect against BSR (hamster renal adenocarcinoma), Hep-2 (Human laryngeal carcinoma), and P815 (murine mastocytoma) in dose-dependent manner [29]. Similar results were obtained by Sulaiman et al. [30] with Iraqi propolis, who described the inhibitory effects against the proliferation of HL-60 and colony potential of HCT116 cells.

Despite the high cytotoxicity displayed by most of the propolis samples against tumor cell lines studied herein, the samples also showed toxicity for nontumour (normal) liver primary culture (PLP2). da Silva Frozza et al. [31] have also investigated the cytotoxicity of extracts of red Brazilian propolis for two tumor cell lines (Hep-2 and HeLa) and human normal epithelial embryonic kidney (Hek-293), reporting also higher IC50 value for Hek-293 when compared to tumor cell lines.

5. Conclusions

Propolis phenolic extracts comprise phytochemicals that should be further studied for their bioactive properties against human colon carcinoma. In the other cases, the proximity of the in vitro cytotoxic doses for tumor and normal cell lines should be taken into consideration and confirmed by in vivo tests. Additional research is needed to determine which compounds are involved in cytotoxicity against normal cells in order to develop new propolis formulations without those compounds to be selectively used against tumor cells.

Conflict of Interests

The authors declare that there is no conflict of interests.

Authors’ Contribution

Ricardo C. Calhelha and Soraia Falcão contributed equally to this work.

Acknowledgments

The authors are grateful to Foundation for Science and Technology (FCT), Portugal, for providing the financial support to the research centers CIMO (PEst-OE/AGR/UI0690/2011) and CQ-UM (PEst-C/QUI/UI0686/2011). Ricardo C. Calhelha thanks FCT, POPH-QREN, and FSE for his Grant (SFRH/BPD/68344/2010). Thanks are also due to the National Federation of Portuguese Beekeepers for supplying propolis samples.

References

  1. M. Carocho and I. C. F. R. Ferreira, “The role of phenolic compounds in the fight against cancer—a review,” Anti-Cancer Agents in Medicinal Chemistry, vol. 13, pp. 1236–1258, 2013. View at Publisher · View at Google Scholar
  2. M. Viuda-Martos, Y. Ruiz-Navajas, J. Fernández-López, and J. A. Pérez-Álvarez, “Functional properties of honey, propolis, and royal jelly,” Journal of Food Science, vol. 73, no. 9, pp. R117–R124, 2008. View at Publisher · View at Google Scholar · View at Scopus
  3. K. Münstedt and S. Bogdanov, “Bee products and their potential use in modern medicine,” Journal of ApiProduct and ApiMedical Science, vol. 1, pp. 57–63, 2009. View at Publisher · View at Google Scholar
  4. G. A. Burdock, “Review of the biological properties and toxicity of bee propolis (propolis),” Food and Chemical Toxicology, vol. 36, no. 4, pp. 347–363, 1998. View at Publisher · View at Google Scholar · View at Scopus
  5. V. C. Toreti, H. H. Sato, G. M. Pastore, and Y. K. Park, “Recent progress of propolis for its biological and chemical compositions and its botanical origin,” Evidence-based Complementary and Alternative Medicine, vol. 2013, Article ID 697390, 13 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  6. M. Marcucci, “Propolis: chemical composition, biological properties and therapeutic activity,” Apidologie, vol. 26, no. 2, pp. 83–99, 1995. View at Google Scholar · View at Scopus
  7. V. S. Bankova, S. L. de Castro, and M. C. Marcucci, “Propolis: recent advances in chemistry and plant origin,” Apidologie, vol. 31, no. 1, pp. 3–15, 2000. View at Google Scholar · View at Scopus
  8. S. I. Falcão, M. Vilas-Boas, L. M. Estevinho, C. Barros, M. R. M. Domingues, and S. M. Cardoso, “Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds,” Analytical and Bioanalytical Chemistry, vol. 396, no. 2, pp. 887–897, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. N. Oršolić, “A review of propolis antitumour action in vivo and in vitro,” Journal of ApiProduct and ApiMedical Science, vol. 2, pp. 1–20, 2010. View at Publisher · View at Google Scholar
  10. M. A. E. Watanabe, M. K. Amarante, B. J. Conti, and J. M. Sforcin, “Cytotoxic constituents of propolis inducing anticancer effects: a review,” Journal of Pharmacy and Pharmacology, vol. 63, no. 11, pp. 1378–1386, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. G. C.-F. Chan, K.-W. Cheung, and D. M.-Y. Sze, “The immunomodulatory and anticancer properties of propolis,” Clinical Reviews in Allergy & Immunology, vol. 44, no. 3, pp. 262–273, 2013. View at Publisher · View at Google Scholar · View at Scopus
  12. V. Bankova, “Chemical diversity of propolis makes it a valuable source of new biologically active compounds,” Journal of ApiProduct and ApiMedical Science, vol. 1, pp. 23–28, 2009. View at Publisher · View at Google Scholar
  13. S. I. Falcão, N. Vale, P. Gomes et al., “Phenolic profiling of Portuguese propolis by LC-MS spectrometry: uncommon propolis rich in flavonoid glycosides,” Phytochemical Analysis, vol. 24, no. 4, pp. 309–318, 2013. View at Publisher · View at Google Scholar · View at Scopus
  14. S. I. Falcão, A. Tomás, N. Vale, P. Gomes, C. Freire, and M. Vilas-Boas, “Phenolic quantification and botanical origin of Portuguese propolis,” Industrial Crops and Products, vol. 49, pp. 805–812, 2013. View at Publisher · View at Google Scholar · View at Scopus
  15. M. J. Valente, A. F. Baltazar, R. Henrique, L. Estevinho, and M. Carvalho, “Biological activities of Portuguese propolis: protection against free radical-induced erythrocyte damage and inhibition of human renal cancer cell growth in vitro,” Food and Chemical Toxicology, vol. 49, no. 1, pp. 86–92, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. I. Valença, F. Morais-Santos, V. Miranda-Gonçalves, A. M. Ferreira, C. Almeida-Aguiar, and F. Baltazar, “Portuguese propolis disturbs glycolytic metabolism of human colorectal cancer in vitro,” BMC Complementary and Alternative Medicine, vol. 13, article 184, 2013. View at Publisher · View at Google Scholar · View at Scopus
  17. R. Guimarães, L. Barros, M. Dueñas et al., “Nutrients, phytochemicals and bioactivity of wild Roman chamomile: a comparison between the herb and its preparations,” Food Chemistry, vol. 136, no. 2, pp. 718–725, 2013. View at Publisher · View at Google Scholar · View at Scopus
  18. C. V. Rao, D. Desai, A. Rivenson, B. Simi, S. Amin, and B. S. Reddy, “Chemoprevention of colon carcinogenesis by phenylethyl-3-methylcaffeate,” Cancer Research, vol. 55, no. 11, pp. 2310–2315, 1995. View at Google Scholar · View at Scopus
  19. T.-H. Leu and M.-C. Maa, “The molecular mechanisms for the antitumorigenic effect of curcumin,” Current Medicinal Chemistry: Anti-Cancer Agents, vol. 2, no. 3, pp. 357–370, 2002. View at Google Scholar · View at Scopus
  20. H. Tapiero, K. D. Tew, G. N. Ba, and G. Mathé, “Polyphenols: do they play a role in the prevention of human pathologies?” Biomedicine & Pharmacotherapy, vol. 56, no. 4, pp. 200–207, 2002. View at Publisher · View at Google Scholar · View at Scopus
  21. T. Nagaoka, A. H. Banskota, Y. Tezuka, I. Saiki, and S. Kadota, “Selective antiproliferative activity of caffeic acid phenethyl ester analogues on highly liver-Metastatic murine colon 26-L5 carcinoma cell line,” Bioorganic & Medicinal Chemistry, vol. 10, no. 10, pp. 3351–3359, 2002. View at Publisher · View at Google Scholar · View at Scopus
  22. A. S. Papathoma, V. Zoumpourlis, A. Balmain, and A. Pintzas, “Suppression of cell transformation and induction of apoptosis by caffeic acid phenethyl ester,” Molecular Carcinogenesis, vol. 31, no. 2, pp. 83–89, 2001. View at Publisher · View at Google Scholar · View at Scopus
  23. B. Etzenhouser, C. Hansch, S. Kapur, and C. D. Selassie, “Mechanism of toxicity of esters of caffeic and dihydrocaffeic acids,” Bioorganic & Medicinal Chemistry, vol. 9, no. 1, pp. 199–209, 2001. View at Publisher · View at Google Scholar · View at Scopus
  24. Y. Akao, H. Maruyama, K. Matsumoto et al., “Cell growth inhibitory effect of cinnamic acid derivatives from propolis on human tumor cell lines,” Biological and Pharmaceutical Bulletin, vol. 26, no. 7, pp. 1057–1059, 2003. View at Google Scholar · View at Scopus
  25. S. Martens and A. Mithöfer, “Flavones and flavone synthases,” Phytochemistry, vol. 66, no. 20, pp. 2399–2407, 2005. View at Publisher · View at Google Scholar · View at Scopus
  26. M. C. Búfalo, J. M. G. Candeias, and J. M. Sforcin, “In vitro cytotoxic effect of Brazilian green propolis on human laryngeal epidermoid carcinoma (HEp-2) cells,” Evidence-Based Complementary and Alternative Medicine, vol. 6, no. 4, pp. 483–487, 2009. View at Publisher · View at Google Scholar
  27. J. M. Sforcin and V. Bankova, “Propolis: is there a potential for the development of new drugs?” Journal of Ethnopharmacology, vol. 133, no. 2, pp. 253–260, 2011. View at Publisher · View at Google Scholar · View at Scopus
  28. T. Kamiya, H. Nishihara, H. Hara, and T. Adachi, “Ethanol extract of Brazilian red propolis induces apoptosis in human breast cancer MCF-7 cells through endoplasmic reticulum stress,” Journal of Agricultural and Food Chemistry, vol. 60, no. 44, pp. 11065–11070, 2012. View at Publisher · View at Google Scholar · View at Scopus
  29. H. A. Mouse, M. Tilaoui, A. Jaafari et al., “Evaluation of the in vitro and in vivo anticancer properties of Moroccan propolisextracts,” Brazilian Journal of Pharmacognosy, vol. 22, no. 3, pp. 558–567, 2012. View at Publisher · View at Google Scholar · View at Scopus
  30. G. M. Sulaiman, A. H. Ad'hiah, K. W. Al-Sammarrae et al., “Assessing the anti-tumour properties of Iraqi propolis in vitro and in vivo,” Food and Chemical Toxicology, vol. 50, no. 5, pp. 1632–1641, 2012. View at Publisher · View at Google Scholar · View at Scopus
  31. C. O. da Silva Frozza, C. S. C. Garcia, G. Gambato et al., “Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis,” Food and Chemical Toxicology, vol. 52, pp. 137–142, 2013. View at Publisher · View at Google Scholar · View at Scopus