Trichinella spiralis Excretory-Secretory Products Protect against Polymicrobial Sepsis by Suppressing MyD88 via Mannose Receptor

Figure 5

TsES protected mice from sepsis via MR. Mice were pretreated with MR siRNA and then underwent the CLP operation. On the day of the operation, MR expression on peritoneal cells was detected by western blot. ^∗𝑃<0.05 compared to the normal group (a). The survival rate was assessed in six groups of mice (𝑛=10 mice/group): (1) PBS + CLP, (2) con siRNA + SLP, (3) MR siRNA + CLP, (4) MR siRNA + TsES + CLP, (5) con siRNA + TsES + CLP, and (6) TsES + CLP. The survival rate was analyzed 4 days after the CLP operation using the log-rank test (b). Twenty-four hours after CLP liver MyD88 expression (c), NF-κB activity (d), and serum cytokine levels (TNF-α, IL-1β, IL-10, and TGFβ1) (e) were determined. Triplicate independent experiments were analyzed. ^∗𝑃<0.05 compared with the PBS + CLP group. ^#𝑃<0.05 compared to the TsES + CLP group.