Oxidative Stress Induces Endothelial Cell Senescence via Downregulation of Sirt6
Knockdown of Sirt6 expression in ECs. ECs were transfected with Sirt6 siRNA (Sirt6-si) or Control siRNA (Con-si). 48 hours after transfection, the protein level of Sirt6 was determined by Western blot and normalized to control (). Tubulin was used as loading control. compared to control.