Oxidative Stress Induces Endothelial Cell Senescence via Downregulation of Sirt6

Figure 6

Knockdown of Sirt6 expression in ECs. ECs were transfected with Sirt6 siRNA (Sirt6-si) or Control siRNA (Con-si). 48 hours after transfection, the protein level of Sirt6 was determined by Western blot and normalized to control (𝑛=3). Tubulin was used as loading control. ^∗𝑃<0.05 compared to control.