Oxidative Stress Induces Endothelial Cell Senescence via Downregulation of Sirt6
Sirt6 overexpression inhibits the activation of senescence pathways induced by H2O2. ECs were infected with adenovirus carrying Sirt6 gene (Ad-Sirt6) or empty vector (control, Ad-Con). At 1 day after transduction, cells were exposed to 75 M H2O2 or vehicle (H2O). At 1 day after H2O2 treatment, cells were lysed and phosphorylated-Rb, p53, and p21 were determined by Western blot. Tubulin was used as loading control. compared with cells infected with Ad-Con and treated with vehicle. compared with cells infected with Ad-Con and treated with H2O2.