Crystallographic Analysis of Ground and Space Thermostable T1 Lipase Crystal Obtained via Counter Diffusion Method Approach

<table>Snapshots of ground-grown crystal (1.1 <svg height="16.4625" id="M46" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 12.4 16.4625" width="12.4" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,16.4)"><path d="M358 762q-41 0 -69.5 29t-28.5 71q0 41 29.5 70t71.5 29q41 0 69 -30q29 -29 29 -70t-29.5 -70t-71.5 -29zM425 862q0 27 -19.5 46t-47.5 19q-26 0 -45 -19.5t-19 -45.5q0 -28 19 -47t45 -19q28 0 47.5 19t19.5 47zM707 19v-19h-255v19q40 0 55 9q14 8 14 24q0 28 -19 70
l-41 94h-262l-46 -114q-9 -22 -9 -42q0 -23 16 -32t54 -9v-19h-199v19q41 4 61 30q17 23 65 137l206 488h20l246 -563q22 -52 39 -70q18 -17 55 -22zM216 257h231l-116 275z" id="xC5"></path></g>
</svg>) and space-grown crystal (1.3 <svg height="16.4625" id="M47" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 12.4 16.4625" width="12.4" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,16.4)"><path d="M358 762q-41 0 -69.5 29t-28.5 71q0 41 29.5 70t71.5 29q41 0 69 -30q29 -29 29 -70t-29.5 -70t-71.5 -29zM425 862q0 27 -19.5 46t-47.5 19q-26 0 -45 -19.5t-19 -45.5q0 -28 19 -47t45 -19q28 0 47.5 19t19.5 47zM707 19v-19h-255v19q40 0 55 9q14 8 14 24q0 28 -19 70
l-41 94h-262l-46 -114q-9 -22 -9 -42q0 -23 16 -32t54 -9v-19h-199v19q41 4 61 30q17 23 65 137l206 488h20l246 -563q22 -52 39 -70q18 -17 55 -22zM216 257h231l-116 275z" id="xC5"></path></g>
</svg>) electron density map countered at 1 sigma level displayed in COOT.</table>

BioMed Research International

fig3

Figure 3

Figure 3: Crystallographic Analysis of Ground and Space Thermostable T1 Lipase Crystal Obtained via Counter Diffusion Method Approach 