Cytoskeleton Modifications and Autophagy Induction in TCam-2 Seminoma Cells Exposed to Simulated Microgravity
Figure 2
RPM exposure and TCam-2 cell apoptosis. (a) Graphical representation of the percentage of apoptotic cell number (anticleaved Caspase-3 positive cells). No differences were observed between TCam-2 cells cultured for 24 hours at 1 g or in RPM culture conditions. On the contrary a slight increase in apoptotic cell percentage is observed after 48 hours of culture. Data are expressed as the mean ± SEM. Same letters indicate no statistical difference. Different letters indicate . (b) Representative images of 1 g (I, III) and RPM (II, IV) exposed TCam-2 cells in 24 (I, II) and 48 (III, IV) hours of culture after cleaved Caspase-3 immunofluorescence. Bar: 50 μm (I and II); 35 μm (III and IV).
