Cytoskeleton Modifications and Autophagy Induction in TCam-2 Seminoma Cells Exposed to Simulated Microgravity
Figure 4
Simulated microgravity influences TCam-2 cell height. (a) Rhodamine-phalloidin staining of TCam-2 cells showing F-actin distribution pattern after 24 hours of culture at 1 g (I) or under RPM (II) conditions. Bar, 20 μm. (b) Representative images of cell height obtained using the Leica confocal software, of samples cultured for 24 hours at 1 g (I) or in RPM (II) conditions. (c) Graphical representation of cell height obtained by confocal microscopy analysis on 1 g and RPM exposed cells after 24 hours of culture (, 10 μm versus 11.0 ± 0.66 μm; ). Data are expressed as the mean ± SEM. (d) Graphical representation of cell height obtained by confocal microscopy analysis on 1 g and RPM exposed cells after 48 hours of culture (13.02 ± 1.32 μm versus 16.02 ± 2.49 μm, resp.). Data are expressed as the mean ± SEM. The values are not statistically significant.
