Successive Nonstatistical and Statistical Approaches for the Improved Antibiotic Activity of Rare Actinomycete Nonomuraea sp. JAJ18

Table 4

Box-Behnken design matrix used in RSM studies along with the experimental and predicted response (antibiotic activity).

Runs	Variables/coded values			Antibiotic activity (U/mL) ± SEM
	Starch	NaCl	MgSO4·7H2O	Experimental	Predicted
1	−	−	0	123.3 ± 5.78	122.94
2	+	−	0	146.6 ± 7.63	145.81
3	−	+	0	121.6 ± 12.6	122.39
4	+	+	0	106.6 ± 16.1	106.96
5	−	0	−	123.3 ± 15.2	123.13
6	+	0	−	131.6 ± 17.5	131.85
7	−	0	+	145.0 ± 15.0	144.75
8	+	0	+	143.3 ± 15.3	143.48
9	0	−	−	135.3 ± 15.0	135.84
10	0	+	−	113.5 ± 15.3	112.89
11	0	−	+	148.6 ± 15.2	149.21
12	0	+	+	133.3 ± 5.78	132.76
13	0	0	0	123.3 ± 15.3	122.62
14	0	0	0	123.3 ± 15.3	122.62
15	0	0	0	121.6 ± 17.6	122.62
16	0	0	0	123.3 ± 23.1	122.62
17	0	0	0	121.6 ± 12.6	122.62