Integrative Analysis of miRNA-mRNA and miRNA-miRNA Interactions
Figure 4
Examples of flexible and selective regulatory network between miRNAs and mRNAs. (a) Selected overexpressed (miR-103a, miR-106b, and miR-194) and underexpressed (miR-15b, miR-100, and miR-125b) miRNAs are used to reconstruct the regulatory network. Their experimentally validated target mRNAs show various expression patterns: some are stably expressed, and others are upregulated or downregulated. Overexpressed miRNAs and mRNAs are here highlighted in red octagons and ellipse, respectively, and underexpressed miRNAs and mRNAs are highlighted in green octagon and ellipse, respectively. Grey ellipses indicate stably expressed mRNAs and mRNAs are not detected in the present study. The targets common to different miRNAs are highlighted in blue rectangles. (b) Selected underexpressed miRNA gene clusters (miR-23b and let-7a-1) and gene families (miR-23 and miR-27) also show complex regulatory networks. These clustered and homologous members are consistently downregulated in tumor cells, and their validated targets show various expression patterns. miRNAs in the let-7a-1 gene cluster are also members of the let-7 gene family. The targets common to these miRNAs have shown upregulated, downregulated, and stable patterns of expression.
