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BioMed Research International
Volume 2014 (2014), Article ID 907915, 8 pages
http://dx.doi.org/10.1155/2014/907915
Research Article

Adalimumab Ameliorates Abdominal Aorta Cross Clamping Which Induced Liver Injury in Rats

1Department of Internal Medicine, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey
2Department of Biochemistry, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey
3Department of Histology and Embryology, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey
4Department of Surgery, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey
5Department of Gastroenterology, School of Medicine, Recep Tayyip Erdogan University, 53100 Rize, Turkey

Received 20 October 2013; Revised 9 December 2013; Accepted 15 December 2013; Published 16 January 2014

Academic Editor: Engin Erturk

Copyright © 2014 Erkan Cure et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to investigate the possible protective effects of adalimumab (ADA) on cell damage in rat liver tissue during ischemia/reperfusion (I/R) injury of infrarenal abdominal aorta. Thirty male Wistar-albino rats were divided into three groups: control, I/R, and I/R+ADA, each group containing 10 animals. Laparotomy without I/R injury was performed in the control group animals. Laparotomy in the I/R group was followed by two hours of infrarenal abdominal aortic cross ligation and then two hours of reperfusion. ADA (50 mg/kg) was administered intraperitoneally as a single dose, to the I/R+ADA group, five days before I/R. The tumor necrosis factor-alpha (TNF-) (pg/mg protein) and nitric oxide (NO) (mol/g protein) levels in the I/R group (430.8 ± 70.1, 8.0 ± 1.1, resp.) were significantly higher than those in the I/R+ADA group (338.0 ± 71.6, ; 6.3 ± 1.2, ) and the control group (345.5 ± 53.3, ; 6.5 ± 1.5, , resp.). I/R causes severe histopathological injury to the liver tissue, but ADA leads to much less histopathological changes. ADA treatment significantly decreased the severity of liver I/R injury. ADA pretreatment may have protective effects on experimental liver injury.