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BioMed Research International
Volume 2014, Article ID 914102, 12 pages
Research Article

Effect of High-Fat Diet upon Inflammatory Markers and Aortic Stiffening in Mice

1Institute of Biomedical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil
2Heart Institute, University of Sao Paulo Medical School, 05403-900 Sao Paulo, SP, Brazil
3Faculty of Pharmaceutical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil
4Health, Biology and Science Center, Mackenzie University, 01302-907 Sao Paulo, SP, Brazil

Received 25 November 2013; Revised 17 March 2014; Accepted 20 March 2014; Published 11 June 2014

Academic Editor: Senthil K. Venugopal

Copyright © 2014 Andre Bento Chaves Santana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Changes in lifestyle such as increase in high-fat food consumption are an important cause for vascular diseases. The present study aimed to investigate the involvement of ACE and TGF-β in the aorta stiffness induced by high-fat diet. C57BL/6 male mice were divided in two groups according to their diet for 8 weeks: standard diet (ST) and high-fat diet (HF). At the end of the protocol, body weight gain, adipose tissue content, serum lipids and glucose levels, and aorta morphometric and biochemical measurements were performed. Analysis of collagen fibers by picrosirius staining of aorta slices showed that HF diet promoted increase of thin (55%) and thick (100%) collagen fibers deposition and concomitant disorganization of these fibers orientations in the aorta vascular wall (50%). To unravel the mechanism involved, myeloperoxidase (MPO) and angiotensin I converting enzyme (ACE) were evaluated by protein expression and enzyme activity. HF diet increased MPO (90%) and ACE (28%) activities, as well as protein expression of ACE. TGF-β was also increased in aorta tissue of HF diet mice after 8 weeks. Altogether, we have observed that the HF diet-induced aortic stiffening may be associated with increased oxidative stress damage and activation of the RAS in vascular tissue.