Differential Macrophage Response to Slow- and Fast-Growing Pathogenic Mycobacteria
Figure 4
Production of reactive oxygen species by THP-1 macrophages after mycobacterial infection. THP-1 macrophages were infected with fast-growing (AB, M. abscessus and FOR, M. fortuitum) or slow-growing mycobacteria (CE, M. celatum and TB, M. tuberculosis), using a MOI of 5. After 6 h of infection, cells were washed and incubated with fresh RPMI medium and 0.1% NBT. Reaction was stopped using 10% SDS in 0.8 N NaOH, and the reduction of NBT was measured at 6 h (a) and 24 h (b). The experiment was performed in triplicate and repeated on three independent occasions. ANOVA with Tukey’s post-test was carried out using a ; NS: nonstimulated cells; PMA: phorbol-12-myristate-13-acetate. Significant difference compared to nonstimulated cells; significant difference compared to M. tuberculosis.
