Enhanced Efficacy of Bleomycin in Bladder Cancer Cells by Photochemical Internalization
Figure 3
Bleomycin hydrolase is strongly expressed in AY-27 cells but the protease inhibitor E-64 does not sensitize AY-27 to bleomycin. (a) Western blots of BLMH expression in K562 (positive control), A431, T24, and AY-27 cells. E-64 treated cells are included as control. PCNA expression level is used as loading control. The image is from one representative experiment out of three. (b) Bleomycin cytotoxicity at two concentrations (5 and 50 IU mL−1) with and without E-64 treatment (concentrations used are listed in Table 1). Cell survival fractions were determined 48 h after 4 h-treatment with the drug(s). The data are from one representative experiment out of three (mean of 24 wells ± SD).
