Multiple Effects of Berberine Derivatives on Colon Cancer Cells
Figure 4
Cell cycle, p53 and p21 expression, and PAR accumulation in cells treated with 10 µM NAXs for 24 h. (a) Cell cycle distribution. A0: apoptotic cells with DNA content <2 C. (b) Immunolocalization of p53 (red fluorescence) and p21 (green fluorescence) in cells treated with NAXs. (c) Western blot analysis of p53 and p21 in cells treated with NAX018 and etoposide. (d) In situ detection of poly(ADP-ribose) (PAR, red fluorescence). Nuclei were counterstained with Hoechst 33258 (blue fluorescence). Scale bar: 50 µm.
