BioMed Research International / 2014 / Article / Fig 6

Research Article

Multiple Effects of Berberine Derivatives on Colon Cancer Cells

Figure 6

Evidence of autophagy. Immunofluorescence (a) and western blot (C) analysis of the autophagosomal marker LC3 II in HCT116 and SW613-B3 cells untreated (C) and treated for 24 h with 10 µM NAX018. As a positive control of autophagy induction, cells were treated with 20 µM HMA [26]. (b) Quantification of cells with autophagic vacuole punctuation; black columns: HCT116, white columns SW613-B3 cells. Effect of the inhibitor 3MA (2.5 mM for 4 h) on the expression of the autophagic marker LC3 (d) and on cell viability evaluated by the MTT assay in HCT116 cells (e). Scale bar: 50 µm. γ-Tubulin was used as protein loading control. .

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