Comparative Analysis of Proliferation and Differentiation Potentials of Stem Cells from Inflamed Pulp of Deciduous Teeth and Stem Cells from Exfoliated Deciduous Teeth
Figure 1
SCIDs formed colonies, expressed CD90, CD105, and CD146, and exhibited cell proliferation similar to SHEDs. (a) HE staining of inflamed primary pulp tissue shows lymphocyte cell infiltration and angiotelectasis. Scale bar: 100 μm. (b) Cells derived from inflamed pulp formed colonies and showed typical fibroblast-like morphology. Scale bar: 10 μm. (c) SHEDs formed colonies and showed typical fibroblast-like morphology. Scale bar: 10 μm. (d) (left column) CD90, (middle column) CD105, and (right column) CD146 were expressed in both SCIDs (top row) and SHEDs (bottom row). (e) Cell growth curves showed that SCIDs and SHEDs had similar growth rates. Cell numbers were counted every two days for one week. The results represent the mean ± standard deviation from three independent experiments. The SCID cell numbers were not significantly different from SHED cell numbers. (f) Cell counting assay showed similar optical density (OD) values for SCIDs and SHEDs at 24 and 48 h. The results represent the mean ± standard deviation from three independent experiments. Student’s -test was performed to determine statistical significance. All error bars represent s.d. (). NS: no significant difference.
