p120 Modulates LPS-Induced NF-κB Activation Partially through RhoA in Bronchial Epithelial Cells
Figure 2
p120 downregulation activates NF-κB signaling pathway in LPS-induced lung inflammation. (a) By immunohistochemical stain, the membranous expression of p120 was significantly reduced in 4-day group compared with the control; some areas showed weakened cytoplasmic p120 expression. Bar = 20 μm. (b) By Western blot, p120 was downregulated in lung lysates of different groups. β-Actin served as internal control. (c) NF-κB expression appeared nuclear distribution by immunohistochemistry (4-day group), compared with NF-κB cytoplasmic expression in the control. Bar = 20 μm. (d) NF-κB p65 expression was increased accompanied with IκBα degradation determined by Western blot. (e) IL-8 secretion was analyzed by ELISA, and it showed gradual increase. Data were expressed as means ± SD (), versus control.
