p120 Modulates LPS-Induced NF-κB Activation Partially through RhoA in Bronchial Epithelial Cells
Figure 4
LPS-induced NF-κB activation was partially inhibited by p120 transfection. (a) 16HBE 14o-cells were transiently transfected with exogenous p120 ΔN and p120 3A for 24 h as well as an empty vector control (mock transfection, MT). p120 ΔN overexpression group was additionally treated with LPS for 60 min. Both NF-κB p65 and RhoA expression remained unchanged in p120 ΔN, p120 3A, and p120 ΔN + LPS groups. (b) Nuclear translocation of NF-κB p65 was not found in p120 ΔN group, but it was detected in p120 ΔN + LPS group. (c, d) Overexpression of p120 reduced IL-8 production in both exogenous p120 3A and p120 ΔN overexpression groups compared with MT group after LPS treatment. Data were expressed as means ± SD (), , and versus MT + No-LPS, p120 ΔN + No-LPS, and p120 3A + No-LPS group, respectively. versus MT + LPS group.
