p120 Modulates LPS-Induced NF-κB Activation Partially through RhoA in Bronchial Epithelial Cells

Figure 5

LPS-induced NF-κB activation was enhanced by p120 knockdown. (a) 16HBE 14o-cells were transfected with p120 siRNA or scrambled siRNA for 48 h, p120 loss significantly increased NF-κB expression, but the total RhoA remained unchanged. (b) Nuclear translocation of p65 was increased. Lamin B served as a nuclear marker, and β-actin served as a cytoplasmic marker. (c, d) IL-8 production was significantly upregulated both with and without LPS treatment. Data were expressed as means ± SD (𝑛=3), ^∗∗∗𝑃<0.001 versus scramble + No-LPS and siRNA + No-LPS, respectively; ^###𝑃<0.001 versus scramble.

(a)

(b)

(c)

(d)