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BioMed Research International
Volume 2014 (2014), Article ID 934848, 12 pages
http://dx.doi.org/10.1155/2014/934848
Research Article

Proteomics and Metabolomics for In Situ Monitoring of Wound Healing

1Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany
2University Center of Orthopedics and Trauma Surgery, University Hospital “Carl Gustav Carus”, TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany
3Department of Oral and Maxillofacial Surgery, University Hospital “Carl Gustav Carus”, TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany
4Department of Metabolomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany
5Institute of Pharmacy, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, 04103 Leipzig, Germany
6Institute of Physiological Chemistry, TU Dresden, Fiedlerstraße 42, 01307 Dresden, Germany
7Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000 Aalborg, Denmark

Received 28 February 2014; Revised 3 June 2014; Accepted 4 June 2014; Published 4 August 2014

Academic Editor: Antonio Salgado

Copyright © 2014 Stefan Kalkhof et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Wound healing of soft tissue and bone defects is a complex process in which cellular differentiation and adaption are regulated by internal and external factors, among them are many different proteins. In contrast to insights into the significance of various single proteins based on model systems, the knowledge about the processes at the actual site of wound healing is still limited. This is caused by a general lack of methods that allow sampling of extracellular factors, metabolites, and proteins in situ. Sampling of wound fluids in combination with proteomics and metabolomics is one of the promising approaches to gain comprehensive and time resolved data on effector molecules. Here, we describe an approach to sample metabolites by microdialysis and to extract proteins simultaneously by adsorption. With this approach it is possible (i) to collect, enrich, and purify proteins for a comprehensive proteome analysis; (ii) to detect more than 600 proteins in different defects including more than 100 secreted proteins, of which many proteins have previously been demonstrated to have diagnostic or predictive power for the wound healing state; and (iii) to combine continuous sampling of cytokines and metabolites and discontinuous sampling of larger proteins to gain complementary information of the same defect.