The K–Cl Cotransporter KCC3 as an Independent Prognostic Factor in Human Esophageal Squamous Cell Carcinoma

Figure 4

KCC3 controlled the cell migration and invasion of esophageal squamous cell carcinoma (ESCC) cells. (a) KCC3 siRNA effectively reduced KCC3 mRNA levels in both TE5 and TE9 cells. Three independent KCC3 siRNAs were investigated to exclude off target effects. (b) The downregulation of KCC3 significantly inhibited cell migration and invasion in TE5 cells. Cell migration and invasion were determined by the Boyden chamber assay. Mean ± SEM; 𝑛=3. ^∗𝑃<0.05: Dunnett’s test (ANOVA: migration; 𝑃<0.0001, invasion; 𝑃<0.0001). (c) The downregulation of KCC3 inhibited cell migration and invasion in TE9 cells. Cell migration and invasion were determined by the Boyden chamber assay. Mean ± SEM; 𝑛=3. ^∗𝑃<0.05: Dunnett’s test (ANOVA: migration; 𝑃<0.0001, invasion; 𝑃=0.0210).

(a)

(b)

(c)