Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts
Table 3
Influence of aECM on mineralisation of osteoblast-precursor cells, premature and mature osteoblasts.
aECM
col
col/sCS1
col/sCS3
col/HA
col/sHA3
hBMSC
OM/D
0.540 ± 0.066
0.778 ± 0.164
0.538 ± 0.082
OM
0.286 ± 0.068
0.355 ± 0.098
0.186 ± 0.049
BM
0.093 ± 0.004
0.044 ± 0.007
0.070 ± 0.007
0.074 ± 0.002
0.096 ± 0.009
MG-63
OM/D
0.043 ± 0.001
0.040 ± 0.002
0.027 ± 0.002
OM
0.019 ± 0.002
0.014 ± 0.001
0.012 ± 0.001
BM
0.003 ± 0.002
0.001 ± 0.001
0.007 ± 0.002
0.009 ± 0.001
0.005 ± 0.003
SaOS-2
OM/D
6.584 ± 0.510
6.360 ± 0.322
7.766 ± 0.964
6.982 ± 0.689
8.495 ± 1.550
OM
6.865 ± 0.639
7.652 ± 0.519
8.504 ± 0.727
7.059 ± 0.393
8.466 ± 1.515
BM
0.058 ± 0.010
0.050 ± 0.058
0.059 ± 0.029
0.068 ± 0.055
0.035 ± 0.011
rCaOB
OM/D
4.408 ± 0.439
5.323 ± 0.526
3.937 ± 0.123
OM
6.552 ± 0.230
6.883 ± 0.244
5.135 ± 0.122
BM
0.075 ± 0.079
0.027 ± 0.006
0.048 ± 0.022
0.003 ± 0.005
0.014 ± 0.003
rBMSC
OM/D
1.625 ± 0.122
1.542 ± 0.129
1.444 ± 0.104
OM
1.559 ± 0.138
1.478 ± 0.086
1.325 ± 0.088
BM
0.009 ± 0.002
0.017 ± 0.005
0.030 ± 0.020
0.021 ± 0.010
0.010 ± 0.002
MC3T3-E1
OM/D
0.288 ± 0.080
0.352 ± 0.101
0.384 ± 0.082
OM
0.384 ± 0.082
0.376 ± 0.098
0.279 ± 0022
BM
0.005 ± 0.002
0.011 ± 0.005
0.006 ± 0.002
0.008 ± 0.003
0.005 ± 0.001
MLO-Y4
OM/D
2.359 ± 0.064
3.045 ± 0.423
3.436 ± 0.126
1.633 ± 0.132
OM
3.436 ± 0.126
7.849 ± 1.348
5.271 ± 0.371
3.783 ± 0.108
4.271 ± 0.108
BM
0.084 ± 0.006
0.028 ± 0.007
0.050 ± 0.014
0.048 ± 0.009
0.009 ± 0.007
Calcium amount mol/cm² given as mean ± SEM; significant differences of OM/D, OM, and BM on (s) GAG versus col-aECM were calculated by two-way ANOVA and indicated with a, b, and c, .
