Interhelical distances between MHC -helices. Each spline is discretised at about 1500 coordinate points. Interhelical distances between spline positions 1, 369, 737, 1105, and 1471 (blue, green, red, cyan, and magenta, resp.,) were evaluated along a 250 ns MD trajectory for three different molecular systems (B4402, B4403, and B4405) and three different polynomial degrees (, (a); , (b); and , (c)).