Figure 6: Frequency dependent CaMKII isoform autophosphorylation in slow oxidative muscle after paced contractions in situ. (a) Example of Thr 287 phosphorylation of CaMKII isoforms in the stimulated (stim) soleus muscle and its contralateral control (rest) after electric stimulation with 150 Hz (Protocol 1) and 10 Hz (Protocol 2). The position of the respective bands is indicated by an arrow. Please note the presence of a second band at the height of the βM isoform in stimulated muscle. (b) Composite figure visualising the fold changes in CaMKII-Thr287 content of CaMKII isoforms for slow and fast motor unit-targeted stimulation for CaMKII isoforms in slow oxidative soleus muscle in a colour code. (c) Line graph resolving the interaction effect of stimulation frequency on Thr287 phosphorylation of the δA-CaMKII isoform. Repeated ANOVA with post hoc test of Fisher, –10.