Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities
Figure 7
Chromatographic and electrophoretic profile of Parawixia bistriata crude venom. (a) Cation exchange chromatography of 2.7 mg of the crude venom of Parawixia bistriata. A 1 mL FF-CM Hytrap (7 × 25 mm) column was used at a flow rate of 0.5 mL/min. Gradient of 0 to 100% of eluent B. Eluent A: 20 mM ammonium bicarbonate, pH 8.0; eluent B: 500 mM ammonium bicarbonate, pH 8.0. Monitoring at 280 nm. (b) SDS-PAGE electrophoresis on 12.5% acrylamide gel of lyophilized fractions from cation exchange chromatography of Parawixia bistriata venom. Coomassie Blue staining. Molecular weight marker: 7–175 kDa.
