A Plant-Produced Antigen Elicits Potent Immune Responses against West Nile Virus in Mice

Figure 1

Western blot analysis of DIII expression in N. benthamiana. DIII was extracted from N. benthamiana leaves and separated on 15% SDS-PAGE gels and blotted onto PVDF membranes. MAb hE16 and a goat anti-human kappa chain antibody were incubated with the membranes sequentially to detect DIII. Lane 1: protein sample extracted from uninfiltrated leaves as a negative control; Lanes 2–5: sample collected 2, 3, 4, and 5 dpi from leaves infiltrated with ER-targeted DIII construct; Lane 6: sample collected 5 dpi from leaves infiltrated with chloroplast-targeted DIII construct; Lane 7: sample collected 5 dpi from cytosol-targeted DIII leaves; Lane 8: E. coli-produced DIII as a positive control.