A Plant-Produced Antigen Elicits Potent Immune Responses against West Nile Virus in Mice
Figure 3
Purification of DIII from N. benthamiana leaves. DIII was purified from leaves infiltrated with ER-targeted DIII construct and analyzed on 15% SDS-PAGE gels and either visualized with Coomassie blue stain (a) or transferred to a PVDF membranes followed by Western analysis with hE16 (b). Lane 1: pH 5.0 precipitation pellet; Lane 2: total extracted protein; Lane 3: pH 5.0 supernatant; Lane 4: Ni IMAC loading; Lane 5: Ni IMAC flow through; Lane 6: Ni IMAC wash; Lane 7: Ni IMAC elute; Lane 8: E. coli-produced DIII; Lane 9: molecular weight marker.
