The Effects of Omega-3 Fatty Acid Supplementation on Dexamethasone-Induced Muscle Atrophy
Figure 3
Means of cross-sectional areas of tibialis anterior muscle fibers (type 1, 2A, and 2B) in rats treated with n-3 PUFA or vehicle for 40 days and dexamethasone or vehicle (last 10 days). (a) Fiber type size distribution; (b) representative mATPase dye histological images of TA muscle from the control group (left), and from the DX + n-3 group (right). Small arrow = type 1 fiber; large arrow = type 2A fiber; asterisk = type 2B fiber. Scale bar (100 μm). CT = control group (), DX = dexamethasone group (), n-3 = n-3 PUFA group (), and DX + n-3 = dexamethasone + n-3 PUFA group (). Values represent means ± S.E.M., analyzed by One-way ANOVA (∗) followed by Bonferroni post hoc test or unpaired Student’s t-test (#), ; ∗∗ or ##, and ∗∗∗ or ###.