Figure 9: CSC-pretreated C. albicans decreased gingival fibroblast growth. Cell cultures were pulsed with CSC-pretreated or untreated C. albicans for 24 or 48 h in a transwell culture system (panel (a)) = Fibroblasts were then trypsinized and viable/cell numbers were determined by trypan blue exclusion assay () (panel (b)) = Culture medium in each condition was used to measure the level of IL-1β by ELISA ().