Review Article

Prospect of Stem Cell Conditioned Medium in Regenerative Medicine

Table 4

Cell number to produce CM per application, volume, and mode of delivery of various cell sources for various conditions and the outcome.

Reference numberCondition/diseaseSpecies Cell source of CMCulture medium/culture type—conditionCell number/applicationVolume and mode of deliveryOutcome

[6] Hind limb ischemia—direct Female athymic mice—20–25 gr Hu-AD-SCαMEM—FBS 10%/monolayer—hypox 1%12.000 40 L—IM—7xGood result
CRM—Hu allo10%/spheroid—hypox 1%48.000Better result
αMEM—FBS 10%/spheroid—hypox 1%Better result

[9]Hind limb ischemia—10 daysMale NOD-SCID mice—10–12 weeksHu-AF-SC–Ckit (+)αMEM—(−)/monolayer—normoxia500.000 80 L—IM—4x Good result

[11]Full thickness wound—5 mm directDiabetic-immunodef. mice—17–23 gHu-UCB-CD34-EPCM199 basal medium (−)/monolayer—normoxia1 × 106100 L—intradermal injectionGood result

[14]Wound
30–50 mm2; 120–140 mm2—48 hours
Male NOD-SCID mice—4-5weeks Hu-BM-MSCαMEM—10% FBS/monolayer—normoxia1 × 108100 L—SC—periphery woundGood result

[17]MCI 48 hoursNude-athymic rat—6–8 weeksHu-BM-MNC-stro-3-MPCαMEM—(−)/monolayer—normoxia1 × 106250 L Intramyocardial Good result

[20]CCl4 injured acute hepatic failure—24 hours SCID-NOD mice—6–8 weeksHu-AF-MSC DMEM—0.5% FBS/monolayer—normoxia1.5 × 106200 L—intrahepatic (left liver lobe) Good result
Hu-AF-MSC- HPLBetter result

[21]Fulminant hepatic failure—24 hoursMale SD rat—250–300 gHu-MSCDMEM—0.05% bovine serum albumin/monolayer—normoxia1.5 × 106 900 L penile veinGood result
Increased survival
[22]Male SD rat—280–370 g Hu-BM-MSCNA—0.05% BSA/monolayer—normoxia2 × 106900 L CM
Penile vein
Good result
Increased survival

[23] Focal cerebral ischemia—72 hours Male SD rat—350–400 g Hu-EDT-SCDMEM (−)/monolayer—normoxia400.00010x10 µL—intranasal (left-right)
Every day D3-D15
Good result
BM-MSC (Lonza)Good result

[24]Ischemic
Stroke—8 days
Male SD mouse—8 weeksHu-AD-MSCαMEM—(−)/spheroid—hypoxia 1%50.400 Infusion 0.5 µL/hour-7 days—lateral ventricleGood result

SCID: severe combined immunodeficiency, NOD: nonobese diabetic, SD: Sprague-Dawley, Hu: human, AD: adipose tissue, SC: stem cell, AF: amniotic fluid, UCB: umbilical cord blood, EPC: endothelial progenitor cell, BM: bone marrow, MSC: mesenchymal SC, MNC: mononuclear cell, MPC: mesenchymal progenitor cell, HPL: hepatic progenitor-like cell, and EDT: exfoliated deciduous tooth.