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BioMed Research International
Volume 2014, Article ID 971345, 14 pages
http://dx.doi.org/10.1155/2014/971345
Research Article

Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement: Generation and Proof-of-Concept

1Division of Virology, Innsbruck Medical University, Peter-Mayr-Straße 4b, 6020 Innsbruck, Austria
2Department of Biotechnology, VIBT, BOKU-University of Natural Resources and Life Sciences, Muthgasse 11, 1190 Vienna, Austria

Received 7 August 2013; Accepted 3 October 2013; Published 12 January 2014

Academic Editor: Lucia Lopalco

Copyright © 2014 Georg Huber et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

When bound to the envelope of viruses, factor H (FH), a soluble regulator of complement activation, contributes to the protection against a potent immune defense mechanism, the complement-mediated lysis (CML). Thus, removing FH from the surface renders viruses, such as HIV, susceptible to CML. For a proof of concept, we developed a construct consisting of recombinant bifunctional single-chain variable fragment (scFv) based on a monoclonal antibody against Friend murine leukemia virus (F-MuLV) envelope protein gp70, which was coupled to specific binding domains (short consensus repeats 19-20; SCR1920) of FH. We used Pichia pastoris as expression system in common shake flasks and optimized expression in high density bench top fermentation. Specific binding of recombinant scFv was proven by flow cytometry. The recombinant scFv-SCR significantly enhanced CML of F-MuLV in vitro implying that FH binding to the viral surface was impaired by the scFv-SCR. This novel concept to enhance virolysis may provide a new approach for antiviral treatment.