Research Article

Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement: Generation and Proof-of-Concept

Figure 6

Relative quantification of viral RNA. F-MuLV was lysed in presence of NMS as a source of complement and SCR-modules in a 100–300-fold molar ratio compared to FH (100, 200, and 300 corresponding to 37 µg, 75 µg, and 112 µg, resp.). Remaining viral RNA was quantified by real-time RT-PCR. Virus-specific RNA was detected by FAM-probes. Relative quantification was performed by the iCylcer’s software.
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