Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement: Generation and Proof-of-Concept

Figure 7

Infectious center assay (ICA). F-MuLV was lysed in presence of NMS as a source of complement and 48scFvSCR1920 in 0.1-, 0.2-, 0.5-, 1- and 2-fold molar ratio compared to FH (corresponding to 0.16 µg, 0.32 µg, 0.8 µg, 1.6 µg and 3.2 µg, resp.). After lysis the amount of remaining infectious viral particles was determined by an ICA. Controls for the single-chain moiety (CtrlscFv:anti-𝛽Gal-scFvSCR1920) and for the SCR-moiety (48scFvSCR1112) were added in 2-fold molar ratio to FH (3.2 µg both). Summary of 6 data-sets was analyzed by using 1-way ANOVA and compared with “Dunnett’s Multiple Comparison Test” performed by GraphPad Prism 5-software.