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BioMed Research International
Volume 2014, Article ID 971345, 14 pages
Research Article

Novel Bifunctional Single-Chain Variable Antibody Fragments to Enhance Virolysis by Complement: Generation and Proof-of-Concept

1Division of Virology, Innsbruck Medical University, Peter-Mayr-Straße 4b, 6020 Innsbruck, Austria
2Department of Biotechnology, VIBT, BOKU-University of Natural Resources and Life Sciences, Muthgasse 11, 1190 Vienna, Austria

Received 7 August 2013; Accepted 3 October 2013; Published 12 January 2014

Academic Editor: Lucia Lopalco

Copyright © 2014 Georg Huber et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supporting information S1 shows the binding of 48scFv and 48scFvSCR1920 including all controls analyzed by FACS. Supporting information S2 illustrates growth curves from shake-flask cultures of Pichia expressing 48scFv or 48scFvSCR1920 under different feeding conditions over time. Supporting information S3 summarizes cell viabilities of Pichia expressing 48scFv or 48scFvSCR1920 after 4d of induction under different conditions.

  1. Supplementary Material