Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 972587, 9 pages
http://dx.doi.org/10.1155/2014/972587
Research Article

Association of Tissue mRNA and Serum Antigen Levels of Members of the Urokinase-Type Plasminogen Activator System with Clinical and Prognostic Parameters in Prostate Cancer

1Urologische Klinik, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91052 Erlangen, Germany
2Klinik für Urologie, Technische Universität Dresden, 01307 Dresden, Germany
3Pathologisches Institut, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91054 Erlangen, Germany
4Department of Urology and Pediatric Urology, Julius-Maximilians-University, 97070 Würzburg, Germany
5Clinic of Urology, Caritas-Hospital St. Josef, 93053 Regensburg, Germany
6Institut für Pathologie, Technische Universität Dresden, 01307 Dresden, Germany
7Department of Obstetrics and Gynecology, Technical University of Munich, 81675 Munich, Germany

Received 13 August 2013; Accepted 21 March 2014; Published 29 April 2014

Academic Editor: Gerhard Unteregger

Copyright © 2014 Omar Al-Janabi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective was to determine the mRNA expression and protein levels of uPA system components in tissue specimens and serum samples, respectively, from prostate cancer (PCa) patients and to assess their association with clinicopathological parameters and overall survival (OS). The mRNA expression levels of uPA, its receptor (uPAR), and its inhibitor type 1 (PAI-1) were analyzed in corresponding malignant and adjacent nonmalignant tissue specimens from 132 PCa patients by quantitative PCR. Preoperative serum samples from 81 PCa patients were analyzed for antigen levels of uPA system members by ELISA. RNA levels of uPA system components displayed significant correlations with each other in the tumor tissues. A significantly decreased uPA mRNA expression in PCa compared to the corresponding nonmalignant tissue was detected. High uPA mRNA level was significantly associated with a high Gleason score. Elevated concentration of soluble uPAR (suPAR) in serum was significantly associated with a poor OS of PCa patients (). PCa patients with high suPAR levels have a significantly higher risk of death (multivariate Cox’s regression analysis; , ). The association of high suPAR levels with poor survival of PCa patients suggests a prognostic impact of suPAR levels in serum of cancer patients.