Targeting the Neddylation Pathway to Suppress the Growth of Prostate Cancer Cells: Therapeutic Implication for the Men’s Cancer

Figure 1

The neddylation pathway was functional and targetable in prostate cancer cells. (a) The components of the neddylation pathway were expressed in prostate cancer cells. Subconfluent cells were subjected to MLN4924 treatment (1 μM) for 12 hours and harvested for immunoblotting (IB) using antibodies against NAE1, UBA3, UBC12, UBE2F, ROC-1, and DCN-1 with GAPDH as a loading control. (b) Neddylation  pathway was activated and targetable in prostate cancer cells. Cells were subjected to MLN4924 (1 μM) for 12 hours and harvested for IB using antibodies against NEDD8 with GAPDH as a loading control. (c) MLN4924 specifically inhibited neddylation pathway. Cells were subjected to MLN4924 (1 μM), Bortezomib (1 μM), or MG132 (20 μM) for 4 hours and harvested for IB using antibodies against NEDD8 and cullin1 with GAPDH as a loading control. MLN, MLN4924; Bort, Bortezomib.