Association of CHMP4B and Autophagy with Micronuclei: Implications for Cataract Formation

Figure 4

Lamp1 and LC3 localize adjacent to micronuclei in HeLa and HLEB-3 cells. (a) Confocal micrographs of HeLa cells stained with CHMP4B, Lamp1, and DAPI. Lamp1 positive lysosomes localize adjacent to CHMP4B positive micronucleus (arrow). Scale bars: 10 μm. Magnification of the micronucleus stained with CHMP4B, Lamp1, and DAPI is shown in the inset. (b) Confocal micrographs of HeLa cells stained with CHMP4B, LC3, and DAPI. CHMP4B positive micronucleus is adjacent to LC3 (arrow). Scale bars: 10 μm. Magnification of the micronuclei stained with CHMP4B, DAPI, and LC3 is shown in the inset. (c)-(d) Confocal micrographs of HLEB-3 cells stained with CHMP4B, Lamin A, and DAPI. CHMP4B is present on the intercellular bridge (c) and the micronucleus (d). Scale bars: 10 μm. (e)-(f) Confocal micrographs of HLEB-3 cells stained with Lamp1, LC3, and DAPI. Lamp1 positive and LC3 positive structures localize adjacent to the micronuclei (arrows). Scale bars: 10 μm.