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BioMed Research International
Volume 2014, Article ID 978582, 8 pages
http://dx.doi.org/10.1155/2014/978582
Research Article

Analgesic, Anti-Inflammatory, and Chondroprotective Activities of Cryptolepis buchanani Extract: In Vitro and In Vivo Studies

1Department of Pharmacology and Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand
2Department of Medical Technology, Faculty of Associated Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand
3Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry and Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand
4Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand
5The Forest Herbarium National Park, Wildlife and Plant Conservation Department, Ministry of Natural Resources and Environment, Pholyothin Road, Bangkok 10900, Thailand

Received 1 May 2014; Accepted 7 July 2014; Published 27 August 2014

Academic Editor: Brad Upham

Copyright © 2014 Nutthiya Hanprasertpong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cryptolepis buchanani Roem. & Schult. is widely used in folk medicine in Southeast Asia for treating muscle tension and arthritis. This study aimed to investigate an analgesic activity of the methanol extract of C. buchanani (CBE) in acetic acid-induced writhing response in mice, and to examine its anti-inflammatory activity in ethyl phenylpropiolate- (EPP-) induced ear edema and carrageenan-induced paw edema in rats. Its effects on cartilage degradation induced by interleukin-1β (IL-1β) in porcine cartilage explant culture were also determined. This study demonstrated that CBE significantly reduced acetic acid-induced writhing response. It also inhibited edema formation in both EPP-induced ear edema and carrageenan-induced paw edema models. In cartilage explant culture, CBE significantly reduced the sulfated glycosaminoglycan and hyaluronan released into culture media while it reserved the uronic acid and collagen within the cartilage tissues. It also suppressed the matrix metalloproteinase-2 activity with no effect on cell viability. In conclusion, CBE shows analgesic, anti-inflammatory, and chondroprotective effects in this preliminary study. Therefore, CBE may be useful as an alternative treatment for osteoarthritis.