BioMed Research International / 2014 / Article / Fig 2

Research Article

The Role of PinX1 in Growth Control of Breast Cancer Cells and Its Potential Molecular Mechanism by mRNA and lncRNA Expression Profiles Screening

Figure 2

Growth control of breast cell lines by PinX1 overexpression and knockdown. (a) MTT assay showed a lower growth rate in the pcDNA3.1-PinX1 transfected MCF-7 cells than the untransfected and vector transfected control cells. (b) MTT assay showed a higher growth rate in the PinX1 siRNA3 tranfected MCF-10A cell line than the untransfected and siRNA NC transfected control cells. (c) Colorimetric focus-formation assay showed pcDNA3.1-PinX1 stable transfected MCF-7 cells had a lower focus counting than the empty vector stably tranfected control cells. (d), (e), and (f) Flow cytometry analysis indicated a G0/G1 phase arrest and S phase inhibition in the pcDNA3.1-PinX1 transfected MCF-7 cells compared to the untransfected and vector transfected control cells. (g), (h), and (i) Flow cytometry analysis indicated a decreased G0/G1 phase and increased S phase rate in the PinX1 knockdown MCF-10A cells compared to the untransfected and siRNA NC transfected control cells.
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