Alkylation of Histidine Residues of Bothrops jararacussu Venom Proteins and Isolated Phospholipases : A Biotechnological Tool to Improve the Production of Antibodies
Figure 5
SPR showing protein interactions with immobilized BthTX-I (a) and BthTX-II (b) assay with native antibodies and antibodies chemically alkylated by p-bromophenacyl bromide (BPB). Concerning antibodies for the native and alkylated BthTX-I and BthTX-II, the assay was conducted at a flow rate of 30 μL/min for 1 min at 37°C. These samples were carried with HBS-P 1X Buffer (GE Healthcare, USA) pH 7.4.
