Remote Dose-Dependent Effects of Dry Needling at Distant Myofascial Trigger Spots of Rabbit Skeletal Muscles on Reduction of Substance P Levels of Proximal Muscle and Spinal Cords
Figure 4
Alterations of substance P (SP) levels at superficial laminae of L5-S2 after one-(1D) and five-dosage (5D) of dry needling at gastrocnemius in experimental (1D and 5D groups) and sham-operated groups (s1D and s5D groups). The representative photomicrographs indicate immunohistochemical labeling for SP in ipsilateral dorsal horns of lumbosacral spinal cords at the timepoints immediately (a) and 5 days (b) after dry needling. Histograms indicate the quantitative analysis of SP immunoreactivity in ipsilateral and contralateral spinal cords (c). represents significant difference () compared with sham-operated groups (s1D and s5D). # represents significant difference () between 1D and 5D groups (scale bar = 100 μm).
