Improvement of the Digestibility of Sulfated Hyaluronans by Bovine Testicular Hyaluronidase: A UV Spectroscopic and Mass Spectrometric Study
Negative ion MALDI-TOF mass spectra of differently sulfated HA samples subsequent to digestion with hyaluronidase but without any further purification of the reaction mixture. Trace (a) corresponds to native HA, while (b) and (c) represent HA with dss = 1.2 and 1.8, respectively. All spectra were recorded in the linear mode of the MS device and in the presence of 9-aminoacridine as matrix with a sample to matrix ratio of 2 to 1 (v/v). This corresponds to a 25-fold weight excess of the matrix. Peaks stemming from impurities are marked by asterisks. Please note that the achievable mass accuracy is only of the order of about 200 ppm. This is a rather typical value if the linear (but not the reflector modus) is used and the reason why only one decimal is given.