Improvement of the Digestibility of Sulfated Hyaluronans by Bovine Testicular Hyaluronidase: A UV Spectroscopic and Mass Spectrometric Study

Figure 5

Dependence of the Reissig absorption on the pH value of the digestion mixture. Since the HA sample with the highest sulfate content (sHA3) was most refractive to the hyaluronidase digestion, only this sample was investigated. All samples were digested with BTH for 20 hours at 37°C and physiological NaCl concentration (0.15 M).